Finalyse®

Finalyse®: A Novel Pre-harvest Hide Wash

OVERVIEW
  • Novel pre-harvest hide wash used to reduce E. coli O157:H7 and other Shiga toxin-producing E. coli (STEC) pathogens in beef cattle
  • Highly effective in reducing E. coli O157:H7 at the plant
  • Contains naturally occurring phages developed specifically to reduce E. coli O157:H7 and other STEC pathogens
  • Safe to workers, equipment and environment
  • Key step in a multi-hurdle food safety intervention program
  • One-liter bottle treats 3,000 head of cattle
MODE OF ACTION

Finalyse® contains a mixture of naturally occurring phages with a unique mode of action that specifically targets E. coli pathogens.

  • Phages in Finalyse use E. coli as host cells to rapidly replicate1
    • Phage replication weakens the E. coli cell wall, which bursts and releases new phages to destroy additional bacteria within 30-40 minutes2
  • Phage attachment compromises an E. coli cell within 5 minutes2
  • Finalyse dwell time is equal to time required for phage to find host cell. A one-hour dwell time equals adequate incubation time for ≥1Log reduction3
EFFICACY

Trials in-lab, in-plant and in simulated commercial settings demonstrate Finalyse efficacy.

Surrogate Model Studies2-4
  • A bacterial culture of a non-virulent E. coli surrogate was grown, diluted and the cell suspension was applied to hide patches to simulate cattle application
  • After attachment period, Finalyse and control water washes were applied to hide patches with a one-hour dwell time
    • Effectively reduced E. coli O157:H7 by ≥1Log after one-hour phage incubation
    • Generated a 1 to 2Log reduction of E. coli O157:H7 compared to tap water control
    • Efficacy was maintained as bacterial levels increased
  • More effective (≥2Log reduction) at room temperature (24°C) but maintains a ≥1Log reduction at temperatures of 38°C
  • Further validation testing verified that E. coli O157:H7 host cells are immediately unviable upon Finalyse phage injection
Prevalence of E. coli on Variety Meats5*
  • Conducted at seven commercial facilities; Finalyse treated cattle compared to same time period previous year and over 3-year average
  • Finalyse reduced E. coli prevalence by 44% compared to previous year
  • Finalyse reduced E. coli prevalence by 37.4% compared to 3-year average
SAFETY1

The Finalyse solution uses naturally occurring phages to destroy harmful bacteria in a natural and safe process.

  • Phages are found in soil and water and will naturally degrade without a host
  • Phages are safely used in a variety of agricultural and food-safety applications because of their attraction to very specific hosts
NOVEL APPLICATION SYSTEM

When used according to label directions, Finalyse and the Finalyse Application SystemTM easily configure to current pre-harvest, hide-on spaces.

  • Applied as an overhead spray system in the holding pens or lairage area spray system
  • Can be added to multiple pre-harvest, hide-on application points both inside and outside the plant

The Passport Food Safety Solutions team will coordinate with plant personnel to help:

  • Identify the best system location
  • Confirm space and utility requirements
  • Install the service system
  • Calibrate and regularly validate titers
  • Train plant personnel

1Information adapted from the OmniLytics website at http://omnilytics.com/home_info/home_info_technology.html.
2Hyde, J. “Optimization and validation of phage treated non-pathogenic E. coli in the beef hide patch model.” Data on file.
3Pillai, S., C. Rambo, and J. McReynolds 2013. “Finalyse Surrogate Research Model.” Data on file.
4Bose, S.K. and R.J. Warren. 1969. “Bacteriophage-induced inhibition of host functions II. Evidence for multiple, sequential bacteriophage-induced deoxyribonucleases responsible for degradation of cellular deoxyribonucleic acid.” J. Virol. 3:549-556.
5Weekly VM-EC 7% Update 2007- 2010 ytd. 2012. Data on file.


Passport Food Safety Solutions
6935 Vista Drive
West Des Moines, IA 50266
515.334.8035

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